How must Tisseel Fibrin Sealant be stored?

• A. Refrigerated at 4°C
• B. Frozen at -20°C; must thaw before use
• C. Room temperature
• D. Freeze-dried at -80°C

Answer: (B)

Tisseel Fibrin Sealant is a biological product whose two protein components must be kept very cold to stay stable. Keeping the vials frozen at about -20°C preserves the activity of fibrinogen and thrombin until you’re ready to use them. Before applying, you thaw them so the components mix properly and can work as intended. Storing at room temperature would risk loss of potency and integrity, and this product isn’t supplied freeze-dried. Therefore, freezing at -20°C with thawing before use is the correct approach.